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Gold ΔMass ✓ Hydroxylation

CYP82P3

Eschscholzia californica CYP82P

Papaveraceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
dihydrosanguinarine
PubChem ↗ CN1CC2=C(C=CC3=C2OCO3)C4=C1C5=CC6=C(C=C5C=C4)OCO6
CYP82P3
ΔMass: +15.9949 Da hydroxylation(+15.9949)
Product
10-hydroxydihydrosanguinarine
PubChem ↗ CN1CC2=C(C3=C1C4=CC5=C(C=C4C=C3)OCO5)C(=CC6=C2OCO6)O

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Recombinant CYP82P3 expressed in Saccharomyces cerevisiae; also forms 10-hydroxydihydrochelerythrine from dihydrochelerythrine.

Reaction & quality gates

Mechanism
Hydroxylation (+15.99 Da)
Evidence type
In vitro
Confidence
High
Data tier
Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class
Alkaloids
Product class
Alkaloids

Literature & public identifiers

PubMed
PMID:29301019 ↗
DOI
10.1093/pcp/pcx210 ↗
UniProt
A0A2Z6BXV9 ↗
GenBank
NCBI TaxID
3467 ↗

Mining of the Uncharacterized Cytochrome P450 Genes Involved in Alkaloid Biosynthesis in California Poppy Using a Draft Genome Sequence.