CYP728B70

Tripterygium wilfordii

Celastraceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

miltiradiene

PubChem ↗ CC(C)C1=CCC2=C(C1)CC[C@@H]3[C@@]2(CCCC3(C)C)C

CYP728B70

ΔMass: +27.9585 Da mismatch

Product

dehydroabietic acid

PubChem ↗ CC(C)C1=CC2=C(C=C1)[C@]3(CCC[C@@]([C@@H]3CC2)(C)C(=O)O)C

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; CYP728B70 overexpression in yeast leads to dehydroabietic acid production; RNAi in T. wilfordii cells reduces triptolide accumulation.

Reaction & quality gates

Mechanism: Oxidation
Evidence type: In vivo
Confidence: High
Data tier: Silver
mass=mismatch
Substrate class: Terpenoids
Product class: Organic acids

Literature & public identifiers

PubMed: PMID:32080175 ↗
DOI: 10.1038/s41467-020-14776-1 ↗
UniProt: —
GenBank: —
NCBI TaxID: 458696 ↗

Genome of Tripterygium wilfordii and identification of cytochrome P450 involved in triptolide biosynthesis.

Evidence trail

Reaction & quality gates

Literature & public identifiers

Other reactions by this enzyme