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Silver mismatch Hydroxylation

CYP81F2

Arabidopsis thaliana CYP81F

Brassicaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
3-indolylmethyl glucosinolate (I3M)
PubChem ↗ C1=CC=C2C(=C1)C(=CN2)C/C(=N/OS(=O)(=O)O)/S[C@H]3[C@@H]([C@H]([C…
CYP81F2
ΔMass: +30.0106 Da mismatch
Product
4-methoxy-3-indolylmethyl glucosinolate (4MOI3M)
PubChem ↗ COC1=CC=CC2=C1C(=CN2)C/C(=N/OS(=O)(=O)O)/S[C@H]3[C@@H]([C@H]([C…

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; 文献描述CYP81F2将I3M转化为4MOI3M,且葡萄糖处理诱导其表达,证据来自体内基因表达分析。

Reaction & quality gates

Mechanism
Hydroxylation
Evidence type
In vivo
Confidence
High
Data tier
Silver
mass=mismatch
Substrate class
Glucosinolates
Product class
Glucosinolates

Literature & public identifiers

PubMed
PMID:27549907 ↗
DOI
10.1038/srep31854 ↗
UniProt
C3W7I0 ↗
GenBank
NCBI TaxID
3702 ↗

Glucose enhances indolic glucosinolate biosynthesis without reducing primary sulfur assimilation.