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Gold ΔMass ✓ Other

CYP71BL2

Lactuca sativa CYP71B

Asteraceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
germacrene A acid
PubChem ↗ C/C/1=C\CC/C(=C/C[C@@H](CC1)C(=C)C(=O)O)/C
CYP71BL2
ΔMass: -2.0157 Da fallback:~-2 Da (成酮/脱氢)
Product
costunolide
PubChem ↗ C/C/1=C\CC/C(=C/[C@@H]2[C@@H](CC1)C(=C)C(=O)O2)/C

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt Other; Other; De novo synthesis of costunolide in engineered yeast expressing LsCOS; spontaneous lactonization after 6β-hydroxylation.

Reaction & quality gates

Mechanism
Ketone formation (-2.02 Da)
Evidence type
In vivo
Confidence
High
Data tier
Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class
Organic acids
Product class
Terpenoids

Literature & public identifiers

PubMed
PMID:21515683 ↗
DOI
10.1074/jbc.m110.216804 ↗
UniProt
F8S1I0 ↗
GenBank
NCBI TaxID
4236 ↗

Lettuce costunolide synthase (CYP71BL2) and its homolog (CYP71BL1) from sunflower catalyze distinct regio- and stereoselective hydroxylations in sesquiterpene lactone metabolism.