CYP76AH15

Coleus forskohlii CYP76A

Lamiaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping

Substrate

miltiradiene

PubChem ↗ CC(C)C1=CCC2=C(C1)CC[C@@H]3[C@@]2(CCCC3(C)C)C

CYP76AH15

ΔMass: +13.9793 Da mismatch

Product

ferruginol

PubChem ↗ CC(C)C1=C(C=C2C(=C1)CC[C@@H]3[C@@]2(CCCC3(C)C)C)O

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Yeast cells expressing CYP76AH15 and producing miltiradiene; product identified by GC-MS and authentic standard.

Reaction & quality gates

Mechanism: Hydroxylation
Evidence type: In vivo
Confidence: High
Data tier: Silver
mass=mismatch
Substrate class: Terpenoids
Product class: Other

Literature & public identifiers

PubMed: PMID:30453976 ↗
DOI: 10.1186/s12934-018-1027-3 ↗
UniProt: KT382358 ↗
GenBank: KT382358 ↗
NCBI TaxID: 41228 ↗

Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast.

Evidence trail

Reaction & quality gates

Literature & public identifiers

Other reactions by this enzyme