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Gold ΔMass ✓ Hydrolysis

CYP81A12

Echinochloa crus CYP81A

Poaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
diclofop-methyl
PubChem ↗ CC(C(=O)OC)OC1=CC=C(C=C1)OC2=C(C=C(C=C2)Cl)Cl
CYP81A12
ΔMass: -14.0157 Da fallback:~-14 Da (脱甲基/脱烷基)
Product
diclofop acid
PubChem ↗ CC(C(=O)O)OC1=CC=C(C=C1)OC2=C(C=C(C=C2)Cl)Cl

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ysis; ysis; CYP81A12 from E. phyllopogon validated through heterologous expression in transformed rice (ref 20).

Reaction & quality gates

Mechanism
Demethylation (-14.01 Da)
Evidence type
In vivo
Confidence
High
Data tier
Gold
Latin binomial + experiment + dual SMILES + ΔMass pass
Substrate class
Other
Product class
Organic acids

Literature & public identifiers

PubMed
PMID:41930952 ↗
DOI
10.1021/acs.jafc.5c16988 ↗
UniProt
R4WPI5 ↗
GenBank
NCBI TaxID
45619 ↗

High Temperatures Are Required for Expression of Metabolic Resistance to Cyhalofop-Butyl in a Biotype of <i>Echinochloa crus-galli</i>.