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Quarantine ΔMass ✓ Oxidation

CYP96T1-LIKE-1

Lycoris aurea CYP96T

Amaryllidaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
4′-O-methylnorbelladine
PubChem ↗ COC1=C(C=C(C=C1)CNCCC2=CC=C(C=C2)O)O
CYP96T1-LIKE-1
ΔMass: -2.0157 Da ketone_formation(-2.0157)
Product
demethylnarwedine
PubChem ↗ [H][C@]12CC(=O)C=C[C@]13CCNCc1ccc(OC)c(c13)O2

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; para-ortho' oxidative coupling; major product; transient expression in N. benthamiana with LC-MS confirmation

Reaction & quality gates

Mechanism
Ketone formation (-2.02 Da)
Evidence type
In vivo
Confidence
High
Data tier
Quarantine
PDF audit quarantine (enzyme_miss_in_pdf;two_core_fields_in_text;review_structured_partial_core). pdf=39416485.pdf; enzyme_in_pdf=miss; issue=review_structured…
Substrate class
Other
Product class
Other

Literature & public identifiers

PubMed
PMID:39416485 ↗
DOI
10.3389/fpls.2024.1438102 ↗
UniProt
GenBank
NCBI TaxID
152838 ↗

Functional characterization of CYP96T1-like cytochrome P450 from <i>Lycoris aurea</i> catalyzing <i>para-para</i>' and <i>para-ortho</i>' oxidative coupling in Amaryllidaceae alkaloids biosynthesis.