← Back to reactions
Silver mismatch Demethylation

CYP51DT

Dioscorea transversa CYP51D

Dioscoreaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
obtusifoliol
PubChem ↗ C[C@H]1[C@@H]2CCC3=C([C@]2(CC[C@@H]1O)C)CC[C@]4([C@]3(CC[C@@H]4…
CYP51DT
ΔMass: -16.0313 Da mismatch
Product
4α-methylergostatrienol
PubChem ↗ C[C@@H]1CCC[C@@]2(C)C1CC[C@H]3[C@@H]4CC[C@H]([C@H](C)C=CC(=C)C(…

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ation; ation; Purified recombinant CYP51Dt with CPRDt and NADPH; product identified by GC-MS.

Reaction & quality gates

Mechanism
Demethylation
Evidence type
In vitro
Confidence
High
Data tier
Silver
mass=mismatch
Substrate class
Other
Product class
Other

Literature & public identifiers

PubMed
PMID:37142228 ↗
DOI
10.1016/j.jbc.2023.104768 ↗
UniProt
GenBank
NCBI TaxID
1518336 ↗

Characterization of the cholesterol biosynthetic pathway in Dioscorea transversa.