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Quarantine mismatch Cyclization

AgKSL1

Aconitum gymnandrum

Ranunculaceae

Substrate reaction site Product reaction site MCS substructure diff highlighting · RDKit atom mapping
Substrate
ent-8,13-copalyl diphosphate
PubChem ↗ C[C@@]12CCCC([C@H]1CC[C@]34[C@H]2C[C@H](CC3)C(=C)C4)(C)C
AgKSL1
ΔMass: +0.0000 Da mismatch
Product
ent-atiserene
PubChem ↗ C[C@@]12CCCC([C@H]1CC[C@]34[C@H]2C[C@H](CC3)C(=C)C4)(C)C

Evidence trail

Excerpts are machine-extracted plain text for biochemical provenance. Follow DOI / PubMed links for the primary literature; publisher figures and PDF images are not displayed here.

Source text excerpt ization; ization; Recombinant protein expressed in E. coli; product identified by GC-MS comparison with IrKSL4.

Reaction & quality gates

Mechanism
Cyclization
Evidence type
In vitro
Confidence
High
Data tier
Quarantine
non-CYP auxiliary enzyme (pathway partner, not cytochrome P450)
Substrate class
Other
Product class
Other

Literature & public identifiers

PubMed
PMID:36362268 ↗
DOI
10.3390/ijms232113463 ↗
UniProt
ON881910 ↗
GenBank
ON881910 ↗
NCBI TaxID
215071 ↗

Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis in <i>Aconitum gymnandrum</i> Maxim.